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mda content quantification  (Elabscience Biotechnology)


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    Elabscience Biotechnology mda content quantification
    Accumulation of ROS and cell death level in <t>rice</t> <t>seedlings</t> from WT and ox lines under drought and salt stress. ( A ) DAB staining, ( B ) NBT staining, and ( C ) Evan’s blue staining. Three-week-old seedlings grown in soil were subjected to stressors, as follows: For drought stress, seedlings were taken out and dried in the air at room temperature for 4 h (DAB and NBT staining) or 6 h (Evan’s blue staining); for salt stress, 200 mM NaCl was added directly to the water reservoir containing the rice seedling pots for 24 h (DAB and NBT staining) or 36 h (Evan’s blue staining). All the treated rice seedlings were re-subjected to the normal water condition for 12 h to recover before staining with 0.2% DAB ( w/v ) solution (24 h), 0.1% ( w/v ) NBT solution (24 h), or 0.25% Evan’s blue ( w/v ) solution (30 min), followed by boiling in 95% ethanol for 4 h and observed under a light microscope with 10× magnification. Non-treated seedlings were used as the control. White bar = 1 cm and black bar = 1 mm. The stained area was converted from three separated microscopic photos using ImageJ Version 1.45p. ( D ) Malondialdehyde <t>(MDA)</t> content in the leaves of WT, ox2-1, and ox2-2 under drought and salt stress. Error bars denote ± SD of three replicates. The p -values were calculated using the Student’s t -test (** p < 0.05 and *** p < 0.01).
    Mda Content Quantification, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 572 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda content quantification/product/Elabscience Biotechnology
    Average 97 stars, based on 572 article reviews
    mda content quantification - by Bioz Stars, 2026-04
    97/100 stars

    Images

    1) Product Images from "Rice Peroxygenase-9 Negatively Regulates Production of Reactive Oxygen Species and Increases Cellular Resistance to Abiotic Stress"

    Article Title: Rice Peroxygenase-9 Negatively Regulates Production of Reactive Oxygen Species and Increases Cellular Resistance to Abiotic Stress

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms26146918

    Accumulation of ROS and cell death level in rice seedlings from WT and ox lines under drought and salt stress. ( A ) DAB staining, ( B ) NBT staining, and ( C ) Evan’s blue staining. Three-week-old seedlings grown in soil were subjected to stressors, as follows: For drought stress, seedlings were taken out and dried in the air at room temperature for 4 h (DAB and NBT staining) or 6 h (Evan’s blue staining); for salt stress, 200 mM NaCl was added directly to the water reservoir containing the rice seedling pots for 24 h (DAB and NBT staining) or 36 h (Evan’s blue staining). All the treated rice seedlings were re-subjected to the normal water condition for 12 h to recover before staining with 0.2% DAB ( w/v ) solution (24 h), 0.1% ( w/v ) NBT solution (24 h), or 0.25% Evan’s blue ( w/v ) solution (30 min), followed by boiling in 95% ethanol for 4 h and observed under a light microscope with 10× magnification. Non-treated seedlings were used as the control. White bar = 1 cm and black bar = 1 mm. The stained area was converted from three separated microscopic photos using ImageJ Version 1.45p. ( D ) Malondialdehyde (MDA) content in the leaves of WT, ox2-1, and ox2-2 under drought and salt stress. Error bars denote ± SD of three replicates. The p -values were calculated using the Student’s t -test (** p < 0.05 and *** p < 0.01).
    Figure Legend Snippet: Accumulation of ROS and cell death level in rice seedlings from WT and ox lines under drought and salt stress. ( A ) DAB staining, ( B ) NBT staining, and ( C ) Evan’s blue staining. Three-week-old seedlings grown in soil were subjected to stressors, as follows: For drought stress, seedlings were taken out and dried in the air at room temperature for 4 h (DAB and NBT staining) or 6 h (Evan’s blue staining); for salt stress, 200 mM NaCl was added directly to the water reservoir containing the rice seedling pots for 24 h (DAB and NBT staining) or 36 h (Evan’s blue staining). All the treated rice seedlings were re-subjected to the normal water condition for 12 h to recover before staining with 0.2% DAB ( w/v ) solution (24 h), 0.1% ( w/v ) NBT solution (24 h), or 0.25% Evan’s blue ( w/v ) solution (30 min), followed by boiling in 95% ethanol for 4 h and observed under a light microscope with 10× magnification. Non-treated seedlings were used as the control. White bar = 1 cm and black bar = 1 mm. The stained area was converted from three separated microscopic photos using ImageJ Version 1.45p. ( D ) Malondialdehyde (MDA) content in the leaves of WT, ox2-1, and ox2-2 under drought and salt stress. Error bars denote ± SD of three replicates. The p -values were calculated using the Student’s t -test (** p < 0.05 and *** p < 0.01).

    Techniques Used: Staining, Light Microscopy, Control



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    mda content quantification  (Elabscience Biotechnology)
    97
    Elabscience Biotechnology mda content quantification
    Accumulation of ROS and cell death level in <t>rice</t> <t>seedlings</t> from WT and ox lines under drought and salt stress. ( A ) DAB staining, ( B ) NBT staining, and ( C ) Evan’s blue staining. Three-week-old seedlings grown in soil were subjected to stressors, as follows: For drought stress, seedlings were taken out and dried in the air at room temperature for 4 h (DAB and NBT staining) or 6 h (Evan’s blue staining); for salt stress, 200 mM NaCl was added directly to the water reservoir containing the rice seedling pots for 24 h (DAB and NBT staining) or 36 h (Evan’s blue staining). All the treated rice seedlings were re-subjected to the normal water condition for 12 h to recover before staining with 0.2% DAB ( w/v ) solution (24 h), 0.1% ( w/v ) NBT solution (24 h), or 0.25% Evan’s blue ( w/v ) solution (30 min), followed by boiling in 95% ethanol for 4 h and observed under a light microscope with 10× magnification. Non-treated seedlings were used as the control. White bar = 1 cm and black bar = 1 mm. The stained area was converted from three separated microscopic photos using ImageJ Version 1.45p. ( D ) Malondialdehyde <t>(MDA)</t> content in the leaves of WT, ox2-1, and ox2-2 under drought and salt stress. Error bars denote ± SD of three replicates. The p -values were calculated using the Student’s t -test (** p < 0.05 and *** p < 0.01).
    Mda Content Quantification, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda content quantification/product/Elabscience Biotechnology
    Average 97 stars, based on 1 article reviews
    mda content quantification - by Bioz Stars, 2026-04
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Accumulation of ROS and cell death level in rice seedlings from WT and ox lines under drought and salt stress. ( A ) DAB staining, ( B ) NBT staining, and ( C ) Evan’s blue staining. Three-week-old seedlings grown in soil were subjected to stressors, as follows: For drought stress, seedlings were taken out and dried in the air at room temperature for 4 h (DAB and NBT staining) or 6 h (Evan’s blue staining); for salt stress, 200 mM NaCl was added directly to the water reservoir containing the rice seedling pots for 24 h (DAB and NBT staining) or 36 h (Evan’s blue staining). All the treated rice seedlings were re-subjected to the normal water condition for 12 h to recover before staining with 0.2% DAB ( w/v ) solution (24 h), 0.1% ( w/v ) NBT solution (24 h), or 0.25% Evan’s blue ( w/v ) solution (30 min), followed by boiling in 95% ethanol for 4 h and observed under a light microscope with 10× magnification. Non-treated seedlings were used as the control. White bar = 1 cm and black bar = 1 mm. The stained area was converted from three separated microscopic photos using ImageJ Version 1.45p. ( D ) Malondialdehyde (MDA) content in the leaves of WT, ox2-1, and ox2-2 under drought and salt stress. Error bars denote ± SD of three replicates. The p -values were calculated using the Student’s t -test (** p < 0.05 and *** p < 0.01).

    Journal: International Journal of Molecular Sciences

    Article Title: Rice Peroxygenase-9 Negatively Regulates Production of Reactive Oxygen Species and Increases Cellular Resistance to Abiotic Stress

    doi: 10.3390/ijms26146918

    Figure Lengend Snippet: Accumulation of ROS and cell death level in rice seedlings from WT and ox lines under drought and salt stress. ( A ) DAB staining, ( B ) NBT staining, and ( C ) Evan’s blue staining. Three-week-old seedlings grown in soil were subjected to stressors, as follows: For drought stress, seedlings were taken out and dried in the air at room temperature for 4 h (DAB and NBT staining) or 6 h (Evan’s blue staining); for salt stress, 200 mM NaCl was added directly to the water reservoir containing the rice seedling pots for 24 h (DAB and NBT staining) or 36 h (Evan’s blue staining). All the treated rice seedlings were re-subjected to the normal water condition for 12 h to recover before staining with 0.2% DAB ( w/v ) solution (24 h), 0.1% ( w/v ) NBT solution (24 h), or 0.25% Evan’s blue ( w/v ) solution (30 min), followed by boiling in 95% ethanol for 4 h and observed under a light microscope with 10× magnification. Non-treated seedlings were used as the control. White bar = 1 cm and black bar = 1 mm. The stained area was converted from three separated microscopic photos using ImageJ Version 1.45p. ( D ) Malondialdehyde (MDA) content in the leaves of WT, ox2-1, and ox2-2 under drought and salt stress. Error bars denote ± SD of three replicates. The p -values were calculated using the Student’s t -test (** p < 0.05 and *** p < 0.01).

    Article Snippet: For MDA content quantification, third leaves of the WT and stress-treated seedlings were collected, and MDA content was extracted and measured by the Malondialdehyde (MDA) Colorimetric Assay Kit (Cat. #E-BC-K025-S, Elabscience, Houston, TX, USA).

    Techniques: Staining, Light Microscopy, Control